Application and precautions of TES free acid

TES free acid, also known as N-tris (hydroxymethyl) methyl-2-aminoethanesulfonic acid, is an organic sulfonic acid, also known as Tris ethanesulfonic acid. It is synthesized from Tris buffer and has a white crystalline powder appearance with good water solubility. The pKa value at room temperature is 7.5, and the suitable buffer range is between pH 6.8 and 8.2. It is commonly used in experiments such as biochemistry, molecular biology, and cell biology. The application of TES free acid 1. Cell culture. TES free acid is used to prepare buffer solutions without calcium and magnesium, which helps to study cell physiology and gene expression, and is therefore commonly used as a buffer for cell culture media. 2. Protein chemistry. TES free acids have relatively low chelating activity, reducing the chance of metal ion contamination that may affect the accuracy of protein determination. Therefore, they are commonly used as buffer solutions in protein chemistry applications. 3. Enzyme analysis. TES free acids have good performance in measuring ATPase activity, with little interference to the reaction and enzyme activity, and can stabilize the pH value of the reaction solution. 4. Electrophoresis experiment. TES free acid improves the separation of protein or nucleic acid by maintaining and stabilizing the pH value of the experimental liquid and providing appropriate ionic strength for the electrophoresis process. It is commonly used in polyacrylamide gel electrophoresis (PAGE), agarose gel electrophoresis and other experiments. Notes on TES free acid TES free acids are sensitive to changes in light and temperature, so it is suitable to store them in a dark and dry environment at room temperature, and attention should also be paid to sealing and moisture-proof. The change in temperature has a certain impact on the buffering ability of TES free acids, so special attention should be paid to this when conducting relevant experimental operations. The pre configured TES free acid solvent needs to be prepared and used immediately to ensure its effectiveness, and the unconfigured TES free acid powder should also be used up within its expiration date. TES free acid is a versatile compound that can be used in various research scenarios. Proper use according to product instructions can make experimental results more accurate. Desheng has a research history of nearly 20 years on biological buffering agents and has extensive experience in producing TES free acids and other products. The product quality is good and the price is affordable. We look forward to your consultation!